Haptoglobin gene polymorphism and oxidative stress effects on ovarian reserve in Beta - Thalassemia major women
نویسندگان
چکیده
Objective: to study the impact of haptoglobin gene polymorphism on iron overload, oxidative stress and antimullerian hormone in BTM women in Egypt. Methods: case-control study. 47 BTM women, aged between 16-26 years and 47 age-matched regularly menstruating women as control. Haptoglobin Hp1/2 gene polymorphism by PCR, Hemoglobin electrophoresis, serum haptoglobin, ferritin, malondialdehyde MDA, FSH, LH, estrogen and ant-mullerian hormone levels were all analyzed. Results: serum ferritin level and Serum MDA, showed a significant higher level in thalassemic group, p<0.001. Significant lower levels of LH, E2 and AMH were found in thalassemic group compared to the control p<0.001. A significant difference (p<0.05) between both groups as regarding distribution of haptoglobin gene polymorphism was detected. In thalassemic group, Hp2-2 was the most frequent. (odds ratio 2.616 at 95% CI, 1.134-6.033). A significantly lower AMH level in patients with Hp2-2 gene polymorphism. Conclusion: beta thalassemic patients are at great risk of oxidative stress. Haptoglobin gene polymorphism has a great impact on the extent of oxidative stress in these patients. BTM patients have lower serum AMH levels than the age -matched control. AMH significantly negatively correlated with serum ferritin and MDA. This was more evident in patients with HP2-2 genotype.
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